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Last updated: 2016/11/27
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CAMK2A polyclonal antibody

  • Catalog # : PAB14145
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of CAMK2A.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human CAMK2A.
  • Sequence:
  • C-PRTAQSEETRVWHR
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 55.3, 54.1
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • Approx 55 and 65 KDa bands observed in human brain (cerebellum) and mouse brain lysates (calculated MW of 55.3 KDa according to human NP_057065.2 and of 54.1 KDa according to human NP_741960.1 and mouse NP_803126.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Western Blot (0.1-0.3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • CAMK2A polyclonal antibody (Cat # PAB14145) (0.1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • CAMK2A polyclonal antibody (Cat # PAB14145) (3.8 ug/mL) staining of paraffin embedded human brain cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 815
  • Protein Accession#:
  • NP_057065.2;NP_741960.1
  • Gene Name:
  • CAMK2A
  • Gene Alias:
  • CAMKA,KIAA0968
  • Gene Description:
  • calcium/calmodulin-dependent protein kinase II alpha
  • Gene Summary:
  • The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • CaM kinase II alpha subunit,CaM-kinase II alpha chain,CaMK-II alpha subunit,CaMKIINalpha,OTTHUMP00000165787,OTTHUMP00000165788,calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha,calcium/calmodulin-dependent protein kinase II alpha-B subunit
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