NDUFS3 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NDUFS3.
Immunogen
A synthetic peptide corresponding to human NDUFS3.
Sequence
C-DTRPTVRPRNDVAHK
Host
Goat
Theoretical MW (kDa)
30.2
Reactivity
Human
Specificity
Approx 28 KDa band observed in human brain (frontal cortex) lysates (calculated MW of 30.2 KDa according to NP_004542.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:4000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NDUFS3 polyclonal antibody (Cat # PAB14140) (0.3 ug/mL) staining of human frontal cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NDUFS3
Entrez GeneID
4722Protein Accession#
NP_004542.1Gene Name
NDUFS3
Gene Alias
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Gene Description
NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death.
Martinvalet D, Dykxhoorn DM, Ferrini R, Lieberman J.
Cell 2008 May; 133(4):681.
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Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death.
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