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Last updated: 2017/2/19
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Dnmt3b polyclonal antibody

  • Catalog # : PAB14125
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Dnmt3b.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of mouse Dnmt3b.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100)
    ChIP (0.2 ug/ChIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP assays were performed using the U-2 OS (human osteosarcoma cell line), Dnmt3b polyclonal antibody (Cat # PAB14125) and optimized PCR primer sets.
    Chromatin sheared from 1x106 cells and 0.2 ug of Dnmt3b antibody or beads only were used per ChIP experiment.
    The IP'd DNA was analysed at two loci : the MLH1 promoter, which is a positive locus for Dnmt3b, and the CDC6 promoter, a negative locus for Dnmt3b.
    Figure shows the recovery expressed as a % of the input DNA.
    Upper : Recovery of the MLH1 promoter by the Dnmt3b antibody or beads only.
    Bottom : Recovery of the CDC6 promoter by the Dnmt3b antibody or beads only.
  • ELISA
  • ELISA
  • ELISA was performed using a serial dilution of Dnmt3b polyclonal antibody (Cat # PAB14125) and crude serum in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 900.
  • Application Image
  • Gene Information
  • Gene Name:
  • Dnmt3b
  • Gene Alias:
  • MGC124407
  • Gene Description:
  • DNA methyltransferase 3B
  • Other Designations:
  • OTTMUSP00000018212,OTTMUSP00000018242,OTTMUSP00000018243,OTTMUSP00000018244,OTTMUSP00000018245,OTTMUSP00000018246,OTTMUSP00000018247,OTTMUSP00000018248
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