Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) with CBX1 polyclonal antibody (Cat # PAB14123) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk. The expected location of CBX1, CBX3, and CBX5 is indicated on the right. The HP1 antibody is not specific for CBX1, but also recognizes CBX3 and CBX5.
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
heterochromatin protein 1-beta,heterochromatin protein p25 beta,modifier 1 protein