H3K79me3 polyclonal antibody
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of H3K79me3 polyclonal antibody (Cat # PAB14122) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest.
By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1 : 10,000.
Dot Blot
To determine the cross reactivity of H3K79me3 polyclonal antibody (Cat # PAB14122) with other histone H3 modifications, a Dot Blot analysis was performed.
Tested histone H3 modifications include mono- and dimethylation of the same lysine and mono-, di- and trimethylation of lysine 9, 27 and 36. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane and detected with the antibody diluted 1 : 50,000.
Figure shows a high specificity for the modification of interest.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of H3K79me3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the trimethylated lysine 79 (H3K79me3).
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
ELISA (1:500-1:1000)
Dot Blot (1:50000)
Western Blot (1:500)
ChIP (5 ug/ChIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of H3K79me3 polyclonal antibody (Cat # PAB14122) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest.
By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1 : 10,000.Dot Blot
To determine the cross reactivity of H3K79me3 polyclonal antibody (Cat # PAB14122) with other histone H3 modifications, a Dot Blot analysis was performed.
Tested histone H3 modifications include mono- and dimethylation of the same lysine and mono-, di- and trimethylation of lysine 9, 27 and 36. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane and detected with the antibody diluted 1 : 50,000.
Figure shows a high specificity for the modification of interest. -
Publication Reference
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Dynamic regulation of histone lysine methylation by demethylases.
Shi Y, Whetstine JR.
Molecular Cell 2007 Jan; 25(1):1.
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Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
Zheng C, Hayes JJ.
The Journal of Biological Chemistry 2003 Apr; 278(26):24217.
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Translating the histone code.
Jenuwein T, Allis CD.
Science 2001 Aug; 293(5532):1074.
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Dynamic regulation of histone lysine methylation by demethylases.
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