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H3 Pan polyclonal antibody

  • Catalog # : PAB14119
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of H3 Pan.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of unmodified histone H3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:400)
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Histone extracts of NB4 (human promyelocytic leukemia) cells, treated with all-trans retinoic acid (ATRA) to induce cell differentiation, were analysed by Western blot using H3 Pan polyclonal antibody (Cat # PAB14119), diluted of 1:500 in TBS-Tween, containing 5% skimmed milk. It shows the results for cells treated with ATRA for 168 h (lane 1) and 24 h (lane 2) and of untreated control cells (lane 3).
  • To determine the titer of the antibody, an ELISA was performed using a serial dilution of H3 Pan polyclonal antibody (Cat # PAB14119), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:400.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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