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Last updated: 2016/10/23
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H3K27me1 polyclonal antibody

  • Catalog # : PAB14115
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of H3K27me1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the monomethylated lysine 27 (H3K27me1).
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100-1:500)
    Dot Blot (1:20000)
    Western Blot (1:1000)
    ChIP (2 ug/ChIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • Western Blot
  • ELISA was performed using a serial dilution of H3K27me1 polyclonal antibody (Cat # PAB14115), crude serum and Flow Through.
    The antigen used was a peptide containing the histone modification of interest.
    By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 11,900.
  • Dot Blot
  • Dot Blot
  • A Dot Blot analysis was performed to test the cross reactivity of H3K27me1 polyclonal antibody (Cat # PAB14115) with peptides containing other modifications of histone H3 and the unmodified H3K27.
    One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane.
    The antibody was used at a dilution of 1 : 20,000. Figure 3 shows a high specificity of the antibody for the modification of interest.
  • Application Image
  • ChIP
  • Western Blot
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