MBD1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Human osteosarcoma cells (U-2 OS) were transfected with an expression vector for TY1-tagged MBD1.
The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the antibody directed against the TY1-tag and with MBD1 polyclonal antibody (Cat # PAB14113) (lane 2), diluted 1 : 2,000 in TBST containing 3% milk powder.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of MBD1 polyclonal antibody (Cat # PAB14113), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution , the titer of the purified antibody was estimated to be 1 : 26,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MBD1.
Immunogen
A mixture of synthetic peptides (conjugated with KLH) that two corresponding to C-terminus and one to N-terminus of human MBD1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:500-1:1000)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Human osteosarcoma cells (U-2 OS) were transfected with an expression vector for TY1-tagged MBD1.
The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the antibody directed against the TY1-tag and with MBD1 polyclonal antibody (Cat # PAB14113) (lane 2), diluted 1 : 2,000 in TBST containing 3% milk powder.Western Blot
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of MBD1 polyclonal antibody (Cat # PAB14113), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution , the titer of the purified antibody was estimated to be 1 : 26,000. -
Gene Info — MBD1
Entrez GeneID
4152Protein Accession#
Q9UIS9Gene Name
MBD1
Gene Alias
CXXC3, PCM1, RFT
Gene Description
methyl-CpG binding domain protein 1
Omim ID
156535Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21. [provided by RefSeq
Other Designations
OTTHUMP00000163504|OTTHUMP00000163506|OTTHUMP00000163507|methyl-CpG binding domain protein 1 isoform PCM1|the regulator of fibroblast growth factor 2 (FGF-2) transcription
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Interactome
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Disease
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