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Last updated: 2016/11/27

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H3K4me3 polyclonal antibody

  • Catalog # : PAB14109
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against H3K4me3.
  • Immunogen:
  • The region of the histone H3 containing the trimethylated lysine 4 (H3K4me3).
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot was performed using histone extracts from HeLa cells (HeLa HE, 15 ug) and H3K4me3 polyclonal antibody (Cat # PAB14109) at dilution 1 : 500 in TBS-Tween + 5% skimmed milk.
  • Dot Blot
  • Dot Blot
  • Dot Blot was performed to test the cross reactivity of H3K4me3 polyclonal antibody (Cat # PAB14109) with the unmodified histone and with other histone modifications.
    Panel A shows the cross reactivity tests with unmodified histone H3 (Lane 1) and mono-, di- and tri-methylated lysine 4 of histone H3 (lanes 2-4). The antibody only recognizes the trimethylated lysine 4 (H3K4me3) (Lane 4).
    Panel B shows the cross reactivity tests with lysines 4, 9 and 27 of histone H3 (Lanes 1-3) and with lysine 20 of histone H4 (Lane 4).
    The antibody mainly recognizes trimethylated lysine 4 of histone H3 (Lane 1), although some cross reaction is observed with trimethylated lysine 20 of histone H4 (Lane 4).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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