ASH2L polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot was performed on HeLa nuclear extract (HeLa NE, 20 ug) using ASH2L polyclonal antibody (Cat # PAB14106) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.
The antibody recognizes endogenous human ASH2 in HeLa nuclear extract.
On the left side, a molecular weight marker is shown.
The arrow indicates the location of the protein of interest.
Western Blot (Cell lysate)
Western blot was performed using ASH2L polyclonal antibody (Cat # PAB14106) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.
The antibody recognizes ectopically expressed FLAG-tagged ASH2L (ASH2L-F) in extracts (Lane 2) and a protein of correct size in nuclear extracts from HeLa cells (Lane 1).
On the left side, a molecular weight marker is shown.
The panel A shows immunostaining using ASH2L polyclonal antibody (Cat # PAB14106), the panel B is the immunostaining of the same western blot with anti-FLAG antibodies.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ASH2L.
Immunogen
Recombinant protein corresponding to human ASH2L.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot was performed on HeLa nuclear extract (HeLa NE, 20 ug) using ASH2L polyclonal antibody (Cat # PAB14106) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.
The antibody recognizes endogenous human ASH2 in HeLa nuclear extract.
On the left side, a molecular weight marker is shown.
The arrow indicates the location of the protein of interest.Western Blot (Cell lysate)
Western blot was performed using ASH2L polyclonal antibody (Cat # PAB14106) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.
The antibody recognizes ectopically expressed FLAG-tagged ASH2L (ASH2L-F) in extracts (Lane 2) and a protein of correct size in nuclear extracts from HeLa cells (Lane 1).
On the left side, a molecular weight marker is shown.
The panel A shows immunostaining using ASH2L polyclonal antibody (Cat # PAB14106), the panel B is the immunostaining of the same western blot with anti-FLAG antibodies. -
Gene Info — ASH2L
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Interactome
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Publication Reference
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Cloning and characterization of ASH2L and Ash2l, human and mouse homologs of the Drosophila ash2 gene.
Ikegawa S, Isomura M, Koshizuka Y, Nakamura Y.
Cytogenetics and Cell Genetics 1999 Jan; 84(3-4):167.
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Cloning and characterization of ASH2L and Ash2l, human and mouse homologs of the Drosophila ash2 gene.
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