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Last updated: 2017/12/17
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L3MBTL polyclonal antibody

  • Catalog # : PAB14105
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against L3MBTL.
  • Immunogen:
  • N-terminus of human L3MBTL.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot was performed on HeLa nuclear extract (HeLa NE) using L3MBTL polyclonal antibody (Cat # PAB14105) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk. The antibody recognizes endogenous L3MBTL1 in HeLa nuclear extract (HeLa NE). The location of L3MBTL1 is indicated with an arrow.
  • Western Blot
  • Western Blot
  • Western blot was performed using L3MBTL polyclonal antibody (Cat # PAB14105) at dilution 1 : 1,000.
    The antibody recognizes the immunogen (GST-L1N) in the ng range (Lanes 1-3).
    L3MBTL polyclonal antibody (Cat # PAB14105) also detects ectopically expressed FLAG-tagged L3MBTL1 (L3MBTL1-F) (Lane 5).
    L3MBTL polyclonal antibody (Cat # PAB14105) also recognizes a protein of the correct size (approx. 100 kDa) (end. L3MBTL1 : endogenous L3MBTL1) in nuclear extract from HeLa (Lane 8) and HEK293 cells (Lane 7), but not in ML-2 cells (Lane 6).
    A negative control (MOCK) is shown (Lane 4).
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation was performed on nuclear extracts containing ectopically expressed FLAG-tagged L3MBTL1 (L3MBTL1-F) using L3MBTL polyclonal antibody (Cat # PAB14105) and an IgG control antibody (IgG Control).
    The presence of L3MBTL1-F in the input (In ; Lane 1), in the precipitated fraction (P) and in the supernatant fraction (SN) was tested by Western Blot using anti-FLAG antibody.
    L3MBTL1-F was efficiently precipitated by L3MBTL polyclonal antibody (Cat # PAB14105) (Lane 3), since there was no L3MBTL1-F present in the supernatant (Lane 5).
    L3MBTL1-F was not precipitated by the control antibody (IgG Control) (Lane 2) and the L3MBTL1 was still present in the supernatant (Lane 4).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • L3MBTL
  • Gene Alias:
  • DKFZp586P1522,FLJ41181,H-L(3)MBT,KIAA0681,L3MBTL1,dJ138B7.3
  • Gene Description:
  • l(3)mbt-like (Drosophila)
  • Gene Summary:
  • This gene encodes the homolog of a protein identified in Drosophila as a suppressor of malignant transformation of neuroblasts and ganglion-mother cells in the optic centers of the brain. This gene product is localized to condensed chromosomes in mitotic cells. Overexpression of this gene in a glioma cell line results in improper nuclear segregation and cytokinesis producing multinucleated cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000031024,l(3)mbt-like,lethal (3) malignant brain tumor l(3)
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