CDC73 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant CDC73.
Immunogen
Recombinant protein corresponding to full length drosophila CDC73.
Host
Rabbit
Reactivity
Flies, Fruit fly, Human
Form
Liquid
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
ChIP
Western Blot (Cell lysate)
Western blot was performed using whole cell extracts (WCE) from drosophila larva (Lane 1) and drosophila adults (Lane 2) and the CDC73 polyclonal antibody (Cat # PAB14104) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.Western Blot (Cell lysate)
Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 ug) and the CDC73 polyclonal antibody (Cat # PAB14104) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk. -
Gene Info — CDC73
Entrez GeneID
79577Protein Accession#
Q6P1J9 (human)Gene Name
CDC73
Gene Alias
C1orf28, FLJ23316, HPT-JT, HRPT2
Gene Description
cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. The protein is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. This protein appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin. Mutations in this gene have been linked to hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism, and parathyroid carcinoma. [provided by RefSeq
Other Designations
hyperparathyroidism 2 (with jaw tumor)|parafibromin
-
Interactome
-
Disease
-
Publication Reference
-
Drosophila Paf1 modulates chromatin structure at actively transcribed genes.
Adelman K, Wei W, Ardehali MB, Werner J, Zhu B, Reinberg D, Lis JT.
Molecular and Cellular Biology 2006 Jan; 26(1):250.
Application:ChIP, WB, Drosophila, Third-instar larvae.
-
The human PAF complex coordinates transcription with events downstream of RNA synthesis.
Zhu B, Mandal SS, Pham AD, Zheng Y, Erdjument-Bromage H, Batra SK, Tempst P, Reinberg D.
Genes & Development 2005 Jul; 19(14):1668.
-
Molecular evidence indicating that the yeast PAF complex is required for transcription elongation.
Rondon AG, Gallardo M, Garcia-Rubio M, Aguilera A.
EMBO Reports 2004 Jan; 5(1):47.
-
Drosophila Paf1 modulates chromatin structure at actively transcribed genes.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com