Rtf1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Drosophila larva whole cell extract (Lane 1) and drosophila adult whole cell extract (Lane 2) were analysed by Western blot using Rtf1 polyclonal antibody (Cat # PAB14103), used at dilution 1 : 1000. Size and location of the protein are indicated.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Rtf1.
Immunogen
Recombinant protein corresponding to full length drosophila Rtf1.
Host
Rabbit
Reactivity
Flies, Fruit fly
Form
Liquid
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Drosophila larva whole cell extract (Lane 1) and drosophila adult whole cell extract (Lane 2) were analysed by Western blot using Rtf1 polyclonal antibody (Cat # PAB14103), used at dilution 1 : 1000. Size and location of the protein are indicated.Immunofluorescence
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Publication Reference
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Drosophila Paf1 modulates chromatin structure at actively transcribed genes.
Adelman K, Wei W, Ardehali MB, Werner J, Zhu B, Reinberg D, Lis JT.
Molecular and Cellular Biology 2006 Jan; 26(1):250.
Application：IF, ChIP, WB, Drosophila, Third-instar larvae.
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The human PAF complex coordinates transcription with events downstream of RNA synthesis.
Zhu B, Mandal SS, Pham AD, Zheng Y, Erdjument-Bromage H, Batra SK, Tempst P, Reinberg D.
Genes & Development 2005 Jul; 19(14):1668.
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Molecular evidence indicating that the yeast PAF complex is required for transcription elongation.
Rondon AG, Gallardo M, Garcia-Rubio M, Aguilera A.
EMBO Reports 2004 Jan; 5(1):47.
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Drosophila Paf1 modulates chromatin structure at actively transcribed genes.
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