LEO1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
HeLa cell lysate was analysed by Western blot using LEO1 polyclonal antibody (Cat # PAB14102), used at dilution 1 : 2000. Size and location of the protein are indicated.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant LEO1.
Immunogen
Recombinant protein corresponding to full length human LEO1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
Western Blot (1:2000)
ChIP (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Cell lysate)
HeLa cell lysate was analysed by Western blot using LEO1 polyclonal antibody (Cat # PAB14102), used at dilution 1 : 2000. Size and location of the protein are indicated. -
Gene Info — LEO1
Entrez GeneID
123169Gene Name
LEO1
Gene Alias
RDL
Gene Description
Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Omim ID
610507Gene Ontology
HyperlinkGene Summary
LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM
Other Designations
Leo1, Paf1/RNA polymerase II complex component, homolog|replicative senescence downregulated leo1-like|senescence downregulated leo1-like
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Interactome
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Publication Reference
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The human PAF complex coordinates transcription with events downstream of RNA synthesis.
Zhu B, Mandal SS, Pham AD, Zheng Y, Erdjument-Bromage H, Batra SK, Tempst P, Reinberg D.
Genes & Development 2005 Jul; 19(14):1668.
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Molecular evidence indicating that the yeast PAF complex is required for transcription elongation.
Rondon AG, Gallardo M, Garcia-Rubio M, Aguilera A.
EMBO Reports 2004 Jan; 5(1):47.
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The human PAF complex coordinates transcription with events downstream of RNA synthesis.
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