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Last updated: 2017/12/17

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H3K9ac polyclonal antibody

  • Catalog # : PAB14098
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of H3K9ac.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the acetylated lysine 9 (H3K9ac).
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • Immunofluorescence (1:200)
    ChIP (2 ug/IP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.1% BSA, 0.01% thimerosal)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ELISA
  • ELISA
  • ELISA was performed using a serial dilution of H3K9ac polyclonal antibody (Cat # PAB14098) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 6,100.
  • Dot Blot
  • Dot Blot
  • A Dot Blot analysis was performed to test the cross reactivity of H3K9ac polyclonal antibody (Cat # PAB14098) with peptides containing other histone modifications and the unmodified H3K9 sequence.
    One hundred to 0.2 pmol of the respective peptides were spotted on a membrane.
    The antibody was used at a dilution of 1 : 2,000.
    Figure shows a high specificity of the antibody for the modification of interest.
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