Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using ZMYND8 polyclonal antibody (Cat # PAB14097) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
ELISA was performed using a serial dilution of ZMYND8 polyclonal antibody (Cat # PAB14097). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 36,000.
The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq
CTCL tumor antigen se14-3,cutaneous T-cell lymphoma associated antigen se14-3,predicted protein of HQ2893,protein kinase C binding protein 1,zinc finger MYND domain containing protein 8