PAPD4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using PAPD4 polyclonal antibody (Cat # PAB14093) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of PAPD4 polyclonal antibody (Cat # PAB14093).
The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 107,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PAPD4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PAPD4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100-1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using PAPD4 polyclonal antibody (Cat # PAB14093) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of PAPD4 polyclonal antibody (Cat # PAB14093).
The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 107,000. -
Gene Info — PAPD4
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