Product Browser

Last updated: 2016/10/23
  • Related Product Showcase
  • By Application

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

H3K9me3S10p polyclonal antibody

  • Catalog # : PAB14091
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of H3K9me3S10p.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the trimethylated lysine 9 and the phosphorylated serine 10 (H3K9me3S10p).
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:1000-1:10000)
    Dot Blot (1:1000)
    Western Blot (1:500)
    ChIP (5 ul/ChIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • Western Blot
  • ELISA was performed using a serial dilution of H3K9me3S10p polyclonal antibody (Cat # PAB14091). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 87,000.
  • Dot Blot
  • Dot Blot
  • A Dot Blot analysis was performed to test the cross reactivity of H3K9me3S10p polyclonal antibody (Cat # PAB14091) with peptides containing other modifications and unmodified sequences of histone H3.
    One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane.
    The antibody was used at a dilution of 1 : 1,000.
    Figure shows a high specificity of the crude serum for the modification of interest.
  • Application Image
  • ChIP
  • Western Blot
  • RSS
  • YouTube
  • Linkedin
  • Facebook