Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using BCL7C polyclonal antibody (Cat # PAB14089) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
ELISA was performed using a serial dilution of BCL7C polyclonal antibody (Cat # PAB14089). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 209,000.
This gene is identified by the similarity of its product to the N-terminal region of BCL7A protein. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. The function of this gene has not yet been determined. [provided by RefSeq