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CHD5 polyclonal antibody

  • Catalog # : PAB14086
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CHD5.
  • Immunogen:
  • A mixture of synthetic peptides (conjugated with KLH) that corresponding N-terminus and to internal region of human CHD5.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:500-1:1000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HeLa cells were transfected with CHD5 and analysed by Western blot.
    Western blot was performed using 15 to 30 ug nuclear extracts and CHD5 polyclonal antibody (Cat # PAB14086) (Dilution at 1 : 1,000).
    Lane 1 shows the result of the untransfected cells, lane 2 of the transfected cells.
    Western blot performed by Sonia Mulero, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
  • Western Blot
  • ELISA
  • ELISA
  • ELISA was performed using a serial dilution of CHD5 polyclonal antibody (Cat # PAB14086).
    The plates were coated with the peptides used for immunization of the rabbit.
    By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 93,500.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot
  • Gene Information
  • Gene Name:
  • CHD5
  • Gene Alias:
  • DKFZp434N231,KIAA0444
  • Gene Description:
  • chromodomain helicase DNA binding protein 5
  • Gene Summary:
  • Changes in gene expression are associated with changes in chromatin structure, which can result from modification of histones by acetylation and methylation. In addition, SWI/SNF proteins can effect chromatin structure and gene expression by causing ATP-dependent disruption of the nucleosome and by enhancing binding of transcription factors. CHD5 belongs to a group of SWI/SNF proteins called CHD proteins, which contain a SWI/SNF-like helicase/ATPase domain, as well as a DNA-binding domain and a chromodomain that directly modifies chromatin structure (Thompson et al., 2003 [PubMed 12592387]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000001137
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