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Western Blot (Transfected lysate)

HeLa cells were transfected with CHD5 and analysed by Western blot.
Western blot was performed using 15 to 30 ug nuclear extracts and CHD5 polyclonal antibody (Cat # PAB14086) (Dilution at 1 : 1,000).
Lane 1 shows the result of the untransfected cells, lane 2 of the transfected cells.
Western blot performed by Sonia Mulero, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.

Western Blot

Enzyme-linked Immunoabsorbent Assay

ELISA was performed using a serial dilution of CHD5 polyclonal antibody (Cat # PAB14086).
The plates were coated with the peptides used for immunization of the rabbit.
By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 93,500.

Entrez GeneID

26038

Protein Accession#

Q8TDI0

Gene Name

CHD5

Gene Alias

DKFZp434N231, KIAA0444

Gene Description

chromodomain helicase DNA binding protein 5

Omim ID

610771

Gene Ontology

Hyperlink

Gene Summary

Changes in gene expression are associated with changes in chromatin structure, which can result from modification of histones by acetylation and methylation. In addition, SWI/SNF proteins can effect chromatin structure and gene expression by causing ATP-dependent disruption of the nucleosome and by enhancing binding of transcription factors. CHD5 belongs to a group of SWI/SNF proteins called CHD proteins, which contain a SWI/SNF-like helicase/ATPase domain, as well as a DNA-binding domain and a chromodomain that directly modifies chromatin structure (Thompson et al., 2003 [PubMed 12592387]).[supplied by OMIM

Other Designations

OTTHUMP00000001137