TASP1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TASP1.
Immunogen
A mixture of synthetic peptides (conjugated with KLH) that two corresponding to N-terminus and one to internal region of human TASP1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:1000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using TASP1 polyclonal antibody (Cat # PAB14081) diluted 1 : 500 in TBS-Tween containing 5% skimmed milk.Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of TASP1 polyclonal antibody (Cat # PAB14081). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 43,500. -
Gene Info — TASP1
Entrez GeneID
55617Gene Name
TASP1
Gene Alias
C20orf13, FLJ20212, MGC39159, dJ585I14.2
Gene Description
taspase, threonine aspartase, 1
Omim ID
608270Gene Ontology
HyperlinkGene Summary
This gene encodes an endopeptidase that cleaves specific substrates following aspartate residues. The encoded protein undergoes posttranslational autoproteolytic processing to generate alpha and beta subunits, which reassemble into the active alpha2-beta2 heterotetramer. It is required to cleave MLL, a protein required for the maintenance of HOX gene expression, and TFIIA, a basal transcription factor. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000030296|taspase 1|threonine aspartase 1
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