TASP1 polyclonal antibody

Catalog # PAB14081

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Size:100 uL
Price: USD $ 428.00
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  • +1-909-264-1399
    +1-909-992-0619
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  • +1-909-992-3401
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Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using TASP1 polyclonal antibody (Cat # PAB14081) diluted 1 : 500 in TBS-Tween containing 5% skimmed milk.

Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA was performed using a serial dilution of TASP1 polyclonal antibody (Cat # PAB14081). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 43,500.

  • Specification

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of TASP1.

    Immunogen

    A mixture of synthetic peptides (conjugated with KLH) that two corresponding to N-terminus and one to internal region of human TASP1.

    Host

    Rabbit

    Reactivity

    Human

    Form

    Liquid

    Recommend Usage

    ELISA (1:1000)
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In serum (0.05% sodium azide)

    Storage Instruction

    Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using TASP1 polyclonal antibody (Cat # PAB14081) diluted 1 : 500 in TBS-Tween containing 5% skimmed milk.

    Enzyme-linked Immunoabsorbent Assay

    ELISA was performed using a serial dilution of TASP1 polyclonal antibody (Cat # PAB14081). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 43,500.
  • Gene Info — TASP1

    Entrez GeneID

    55617

    Gene Name

    TASP1

    Gene Alias

    C20orf13, FLJ20212, MGC39159, dJ585I14.2

    Gene Description

    taspase, threonine aspartase, 1

    Omim ID

    608270

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes an endopeptidase that cleaves specific substrates following aspartate residues. The encoded protein undergoes posttranslational autoproteolytic processing to generate alpha and beta subunits, which reassemble into the active alpha2-beta2 heterotetramer. It is required to cleave MLL, a protein required for the maintenance of HOX gene expression, and TFIIA, a basal transcription factor. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq

    Other Designations

    OTTHUMP00000030296|taspase 1|threonine aspartase 1

  • Interactome
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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