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Last updated: 2017/1/15
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Mecp2 polyclonal antibody

  • Catalog # : PAB14073
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Mecp2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH at N-terminus) corresponding to N-terminus of mouse Mecp2.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:500-1:5000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Nuclear extracts of mouse fibroblasts (NIH/3T3, 40 ug) were analysed by Western blot using Mecp2 polyclonal antibody (Cat # PAB14073) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
  • ELISA
  • ELISA
  • ELISA was performed using a serial dilution of Mecp2 polyclonal antibody (Cat # PAB14073).
    The wells were coated with the peptide used for immunisation of the rabbit.
    By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 136,000.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • Mecp2
  • Gene Alias:
  • 1500041B07Rik,BB130002,D630021H01Rik,Mbd5,WBP10
  • Gene Description:
  • methyl CpG binding protein 2
  • Other Designations:
  • OTTMUSP00000019241,OTTMUSP00000019680
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