H3K9acS10p polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of H3K9acS10p polyclonal antibody (Cat # PAB14069). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 89,000.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of H3K9acS10p.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the acetylated lysine 9 and the phosphorylated serine 10 (H3K9acS10p).
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:1000-1:4000)
Dot Blot (1:20000)
Western Blot (1:250)
ChIP (15 ul/ChIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
ChIP
Western Blot
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of H3K9acS10p polyclonal antibody (Cat # PAB14069). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 89,000.Dot Blot
-
Publication Reference
-
Dynamic regulation of histone lysine methylation by demethylases.
Shi Y, Whetstine JR.
Molecular Cell 2007 Jan; 25(1):1.
-
Stimulation of the Ras-MAPK pathway leads to independent phosphorylation of histone H3 on serine 10 and 28.
Dunn KL, Davie JR.
Oncogene 2005 May; 24(21):3492.
-
Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
Zheng C, Hayes JJ.
The Journal of Biological Chemistry 2003 Apr; 278(26):24217.
-
Dynamic regulation of histone lysine methylation by demethylases.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com