Mll4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with Mll4 polyclonal antibody (Cat # PAB14067), diluted 1 : 500 in PBS-Tween containing 5% skimmed milk.
Cells homozygous for the targeted mll4 allele (-/-, Lane 2 and 3) show a complete loss of Mll4 protein, whereas the Mll4 protein was detected in flp-recombined ES cells (F/F, Lane 4) and heterozygously targeted ES cells (+/-, Lane 1).
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of Mll4 polyclonal antibody (Cat # PAB14067). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 15,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Mll4.
Immunogen
A mixture of synthetic peptides (conjugated with KLH) that corresponding to internal region of mouse Mll4.
Host
Rabbit
Reactivity
Mouse
Form
Liquid
Recommend Usage
ELISA (1:100-1:500)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with Mll4 polyclonal antibody (Cat # PAB14067), diluted 1 : 500 in PBS-Tween containing 5% skimmed milk.
Cells homozygous for the targeted mll4 allele (-/-, Lane 2 and 3) show a complete loss of Mll4 protein, whereas the Mll4 protein was detected in flp-recombined ES cells (F/F, Lane 4) and heterozygously targeted ES cells (+/-, Lane 1).Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of Mll4 polyclonal antibody (Cat # PAB14067). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 15,000.
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