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Last updated: 2017/8/20

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Hira polyclonal antibody

  • Catalog # : PAB14064
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Hira.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to internal region of mouse Hira.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100-1:200)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot was performed on whole cell lysates from mouse fibroblasts (Lane 1, NIH/3T3) and embryonic stem cells (Lane 2, E14Tg2a) with Hira polyclonal antibody (Cat # PAB14064), diluted 1 : 1,000 in BSA/PBS-Tween.
  • ELISA
  • ELISA
  • ELISA was performed using a serial dilution of Hira polyclonal antibody (Cat # PAB14064).
    The wells were coated with the peptide used for immunisation of the rabbit.
    By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 6,300.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • Hira
  • Gene Alias:
  • AA138857,D16Ertd95e,N28177,Tuple1
  • Gene Description:
  • histone cell cycle regulation defective homolog A (S. cerevisiae)
  • Other Designations:
  • TUP1-like enhancer of split gene 1,histone cell cycle regulation defective homolog A
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