Brd2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot was performed on whole cell lysates from mouse fibroblasts (Lane 1, NIH/3T3) and embryonic stem cells (Lane 2, E14Tg2a) with Brd2 polyclonal antibody (Cat # PAB14061), diluted 1 : 1,000 in BSA/PBS-Tween.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of Brd2 polyclonal antibody (Cat # PAB14061). By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 5,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Brd2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of mouse Brd2.
Host
Rabbit
Reactivity
Mouse
Form
Liquid
Recommend Usage
ELISA (1:50-1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot was performed on whole cell lysates from mouse fibroblasts (Lane 1, NIH/3T3) and embryonic stem cells (Lane 2, E14Tg2a) with Brd2 polyclonal antibody (Cat # PAB14061), diluted 1 : 1,000 in BSA/PBS-Tween.Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of Brd2 polyclonal antibody (Cat # PAB14061). By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 5,000. -
Gene Info — Brd2
Entrez GeneID
14312Protein Accession#
P25440 (human)Gene Name
Brd2
Gene Alias
AW228947, D17H6S113E, Frg-1, Fsrg1, KIAA4005, NAT, Ring3, Rnf3, mKIAA4005
Gene Description
bromodomain containing 2
Gene Ontology
HyperlinkOther Designations
female sterile homeotic-related gene 1
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