H3R17me2 polyclonal antibody
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More Files
- More Functions
ChIP
ChIP assays were performed using human osteosarcoma (U-2 OS) cells, the polyclonal antibody against H3R17me2(asym) (Cat # PAB14059) and optimized PCR primer sets for qPCR.
Figure A: qPCR performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene.
Figure B: qPCR performed with primers for the promoter of the active ALDOA gene and for the coding region of the inactive MYOD gene.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of H3R17me2 polyclonal antibody (Cat # PAB14059).
The antigen used was a peptide containing the histone modification of interest.
By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 40,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of H3R17me2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the asymmetrically dimethylated arginine 17 (H3R17me2 (asym)).
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:1000-1:3000)
Dot Blot (1:20000)
Western Blot (1:250)
ChIP (10-15 ul/ChIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using human osteosarcoma (U-2 OS) cells, the polyclonal antibody against H3R17me2(asym) (Cat # PAB14059) and optimized PCR primer sets for qPCR.
Figure A: qPCR performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene.
Figure B: qPCR performed with primers for the promoter of the active ALDOA gene and for the coding region of the inactive MYOD gene.Western Blot
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of H3R17me2 polyclonal antibody (Cat # PAB14059).
The antigen used was a peptide containing the histone modification of interest.
By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 40,000.Dot Blot
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Publication Reference
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Dynamic regulation of histone lysine methylation by demethylases.
Shi Y, Whetstine JR.
Molecular Cell 2007 Jan; 25(1):1.
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Stimulation of the Ras-MAPK pathway leads to independent phosphorylation of histone H3 on serine 10 and 28.
Dunn KL, Davie JR.
Oncogene 2005 May; 24(21):3492.
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Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
Zheng C, Hayes JJ.
The Journal of Biological Chemistry 2003 Apr; 278(26):24217.
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Dynamic regulation of histone lysine methylation by demethylases.
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