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Last updated: 2016/10/16
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H3R17me2 polyclonal antibody

  • Catalog # : PAB14059
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of H3R17me2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the asymmetrically dimethylated arginine 17 (H3R17me2 (asym)).
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:1000-1:3000)
    Dot Blot (1:20000)
    Western Blot (1:250)
    ChIP (10-15 ul/ChIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • ChIP
  • ChIP assays were performed using human osteosarcoma (U-2 OS) cells, the polyclonal antibody against H3R17me2(asym) (Cat # PAB14059) and optimized PCR primer sets for qPCR.
    Figure A: qPCR performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene.
    Figure B: qPCR performed with primers for the promoter of the active ALDOA gene and for the coding region of the inactive MYOD gene.
  • Western Blot
  • ELISA was performed using a serial dilution of H3R17me2 polyclonal antibody (Cat # PAB14059).
    The antigen used was a peptide containing the histone modification of interest.
    By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 40,000.
  • Dot Blot
  • Application Image
  • Western Blot
  • Dot Blot
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