H3K27me3S28p polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of H3K27me3S10p.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p).
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100-1:500)
Dot Blot (1:20000)
Western Blot (1:250)
Immunofluorescence (1:200)
Immunoprecipitation (5 ul/IP)
ChIP (1 ul/ChIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
ChIP
Western Blot
Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of H3K27me3S28p polyclonal antibody (Cat # PAB14058).
The antigen usedwas a peptide containing the histone modifications of interest.
By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 8,300.Dot Blot
A Dot Blot analysis was performed to test the cross reactivity of H3K27me3S28p polyclonal antibody (Cat # PAB14058) with peptides containing other modifications of histone H3 and H4 and with peptides containing unmodified sequences from histone H3.
One hundred to 0.2 pmol of the peptides were spotted on a membrane.
The antibody was used at a dilution of 1 : 20,000.
Figure shows a high specificity of the crude serum for the peptide containing the modifications of interest. -
Publication Reference
-
Dynamic regulation of histone lysine methylation by demethylases.
Shi Y, Whetstine JR.
Molecular Cell 2007 Jan; 25(1):1.
-
Stimulation of the Ras-MAPK pathway leads to independent phosphorylation of histone H3 on serine 10 and 28.
Dunn KL, Davie JR.
Oncogene 2005 May; 24(21):3492.
-
Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
Zheng C, Hayes JJ.
The Journal of Biological Chemistry 2003 Apr; 278(26):24217.
-
Dynamic regulation of histone lysine methylation by demethylases.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com