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Last updated: 2017/12/10
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H3K27me3S28p polyclonal antibody

  • Catalog # : PAB14058
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of H3K27me3S10p.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p).
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100-1:500)
    Dot Blot (1:20000)
    Western Blot (1:250)
    Immunofluorescence (1:200)
    Immunoprecipitation (5 ul/IP)
    ChIP (1 ul/ChIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • Western Blot
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA was performed using a serial dilution of H3K27me3S28p polyclonal antibody (Cat # PAB14058).
    The antigen usedwas a peptide containing the histone modifications of interest.
    By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 8,300.
  • Dot Blot
  • Dot Blot
  • A Dot Blot analysis was performed to test the cross reactivity of H3K27me3S28p polyclonal antibody (Cat # PAB14058) with peptides containing other modifications of histone H3 and H4 and with peptides containing unmodified sequences from histone H3.
    One hundred to 0.2 pmol of the peptides were spotted on a membrane.
    The antibody was used at a dilution of 1 : 20,000.
    Figure shows a high specificity of the crude serum for the peptide containing the modifications of interest.
  • Application Image
  • ChIP
  • Western Blot
  • Immunofluorescence
  • Immunoprecipitation
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