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Last updated: 2017/12/10
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H3K79me1 polyclonal antibody

  • Catalog # : PAB14056
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of H3K79me1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the monomethylated lysine 79 (H3K79me1).
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:500-1:1000)
    Dot Blot (1:100000)
    Western Blot (1:1000)
    ChIP (5-10 ul/ChIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In serum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • Western Blot
  • ELISA was performed using a serial dilution of H3K79me1 polyclonal antibody (Cat # PAB14056) in antigen coated wells.
    The antigen used was a peptide containing the histone modification of interest.
    By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 30,000.
  • Dot Blot
  • Dot Blot
  • To determine the cross reactivity of H3K79me1 polyclonal antibody (Cat # PAB14056) with other histone H3 modifications, a Dot Blot analysis was performed.
    Tested histone H3 modifications include di- and trimethylation of the same lysine and mono-, di- and trimethylation of lysine 9, 27 and 36. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane and detected with the crude serum diluted 1 : 100,000.
    Figure shows a high specificity for the modification of interest.
  • Application Image
  • ChIP
  • Western Blot
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