Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 ug) with PADI4 polyclonal antibody (Cat # PAB14053), diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
ELISA was performed using a serial dilution of PADI4 polyclonal antibody (Cat # PAB14053) in antigen coated wells. By plotting the absorbance against the crude serum dilution, the titer of the antibody was estimated to be 1 : 40,200.
This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq
HL-60 PAD,OTTHUMP00000002482,PADI-H protein,peptidyl arginine deiminase, type V,protein-arginine deiminase