Dnmt3b polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using Dnmt3b polyclonal antibody (Cat # PAB14046) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of Dnmt3b polyclonal antibody (Cat # PAB14046).
By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 13,500.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Dnmt3b.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of mouse Dnmt3b.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
ELISA (1:100-1:500)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using Dnmt3b polyclonal antibody (Cat # PAB14046) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of Dnmt3b polyclonal antibody (Cat # PAB14046).
By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 13,500. -
Gene Info — Dnmt3b
Entrez GeneID
13436Protein Accession#
Q9UBC3 (human)Gene Name
Dnmt3b
Gene Alias
MGC124407
Gene Description
DNA methyltransferase 3B
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000018212|OTTMUSP00000018242|OTTMUSP00000018243|OTTMUSP00000018244|OTTMUSP00000018245|OTTMUSP00000018246|OTTMUSP00000018247|OTTMUSP00000018248
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