Nanog polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis from mouse embryonic stem (ES) cells with Nanog polyclonal antibody (Cat # PAB13693).
Immunofluorescence
Immunofluorescence with Nanog polyclonal antibody (Cat # PAB13693). Feeder cells are indicated by dotted line circle.
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Specification
Product Description
Rabbit polyclonal antibody raised against Nanog.
Immunogen
Mouse Nanog protein.
Host
Rabbit
Reactivity
Mouse
Specificity
Recognizes mouse Nanog. Cross reactivity with monkey and human observed in immunocytochemistry. Detects bands at 37-44 KDa.
Form
Liquid
Recommend Usage
Immunocytochemistry (1:150-1:700)
Western Blot (1:300-1:2000)
Immunoprecipitation (1:300)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis from mouse embryonic stem (ES) cells with Nanog polyclonal antibody (Cat # PAB13693).Immunocytochemistry
Immunofluorescence
Immunofluorescence with Nanog polyclonal antibody (Cat # PAB13693). Feeder cells are indicated by dotted line circle.Immunoprecipitation
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Gene Info — Nanog
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Publication Reference
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Nanog expression in mouse germ cell development.
Hansen PB, Jensen BL, Yamaguchi S, Andreasen D, Kimura H, Friis UG, Tada M, Skott O, Nakatsuji N, Tada T.
Gene Expression Patterns 2005 Jun; 5(5):639.
Application：IF, IHC-Fr, Mouse, Mouse embryos.
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Pluripotential competence of cells associated with Nanog activity.
Hatano SY, Tada M, Kimura H, Yamaguchi S, Kono T, Hussain SP, Nakano T, Hollstein MH, Suemori H, Harris CC, Nakatsuji N, Tada T.
Mechanisms of Development 2005 Jan; 122(1):67.
Application：IF, WB, Human, Mouse, Embryonic stem cells, NIH/3T3 cells, Thymocytes.
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Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells.
Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, Smith A.
Cell 2003 May; 113(5):643.
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Nanog expression in mouse germ cell development.
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