NR1H3 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of cell and tissue lysates with NR1H3 polyclonal antibody (Cat # PAB13685).
Lane 1 : Rat liver tissue.
Lane 2 : Rat liver tissue.
Lane 3 : MCF-7.
Lane 4 : HeLa.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NR1H3.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human NR1H3.
Host
Rabbit
Theoretical MW (kDa)
50, 43
Reactivity
Human, Mouse, Rat
Specificity
Identical to the related rat and mouse sequence.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of cell and tissue lysates with NR1H3 polyclonal antibody (Cat # PAB13685).
Lane 1 : Rat liver tissue.
Lane 2 : Rat liver tissue.
Lane 3 : MCF-7.
Lane 4 : HeLa. -
Gene Info — NR1H3
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Interactome
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Pathway
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Disease
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Publication Reference
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LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor.
Zelcer N, Hong C, Boyadjian R, Tontonoz P.
Science 2009 Jun; 325(5936):100.
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The nuclear receptor LXR is a glucose sensor.
Mitro N, Mak PA, Vargas L, Godio C, Hampton E, Molteni V, Kreusch A, Saez E.
Nature 2006 Dec; 445(7124):219.
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Reciprocal regulation of inflammation and lipid metabolism by liver X receptors.
Joseph SB, Castrillo A, Laffitte BA, Mangelsdorf DJ, Tontonoz P.
Nature Medicine 2003 Jan; 9(2):213.
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LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor.
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