CASP8 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of cell and tissue lysates with CASP8 polyclonal antibody (Cat # PAB13681).
Lane 1 : Rat thymus tissue.
Lane 2 : Rat liver tissue.
Lane 3 : MCF-7.
Lane 4 : HeLa.
Lane 5 : Smmc.
Lane 6 : Jurkat.
Lane 7 : Colo 320.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CASP8.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human CASP8.
Host
Rabbit
Theoretical MW (kDa)
55.3
Reactivity
Human, Mouse, Rat
Specificity
Different to the related mouse sequence by a single amino acids.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of cell and tissue lysates with CASP8 polyclonal antibody (Cat # PAB13681).
Lane 1 : Rat thymus tissue.
Lane 2 : Rat liver tissue.
Lane 3 : MCF-7.
Lane 4 : HeLa.
Lane 5 : Smmc.
Lane 6 : Jurkat.
Lane 7 : Colo 320. -
Gene Info — CASP8
Entrez GeneID
841Gene Name
CASP8
Gene Alias
ALPS2B, CAP4, FLICE, FLJ17672, MACH, MCH5, MGC78473
Gene Description
caspase 8, apoptosis-related cysteine peptidase
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq
Other Designations
FADD-homologous ICE/CED-3-like protease|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|Mch5 isoform alpha|OTTHUMP00000163718|OTTHUMP00000163719|OTTHUMP00000163720|caspase 8|caspase 8, apoptosis-related cysteine protease|cysteine protease|procaspase-8
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Interactome
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Pathway
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Disease
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Publication Reference
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Pleiotropic defects in lymphocyte activation caused by caspase-8 mutations lead to human immunodeficiency.
Chun HJ, Zheng L, Ahmad M, Wang J, Speirs CK, Siegel RM, Dale JK, Puck J, Davis J, Hall CG, Skoda-Smith S, Atkinson TP, Straus SE, Lenardo MJ.
Nature 2002 Sep; 419(6905):395.
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Structure and chromosome localization of the human CASP8 gene.
Grenet J, Teitz T, Wei T, Valentine V, Kidd VJ.
Gene 1999 Jan; 226(2):225.
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Pleiotropic defects in lymphocyte activation caused by caspase-8 mutations lead to human immunodeficiency.
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