IFT57 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
IFT57 polyclonal antibody (Cat # PAB13672) (1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of IFT57.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human IFT57.
Sequence
C-EQPQEYDDPNATISN
Host
Goat
Theoretical MW (kDa)
49.1
Reactivity
Human, Mouse
Specificity
Approximately 49 KDa band observed in human and mouse brain lysates (calculated MW of 49.1 KDa according to human NP_060480.1 and of 48.8 KDa according to mouse NP_082956.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
IFT57 polyclonal antibody (Cat # PAB13672) (1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — IFT57
Entrez GeneID
55081Protein Accession#
NP_060480.1Gene Name
IFT57
Gene Alias
ESRRBL1, FLJ10147, HIPPI, MHS4R2
Gene Description
intraflagellar transport 57 homolog (Chlamydomonas)
Omim ID
606621Gene Ontology
HyperlinkOther Designations
HIP1 protein interactor|estrogen-related receptor beta like 1|huntingtin interacting protein-1 interacting protein
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Interactome
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Publication Reference
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Crystal structure at 2.8 A of Huntingtin-interacting protein 1 (HIP1) coiled-coil domain reveals a charged surface suitable for HIP1 protein interactor (HIPPI).
Niu Q, Ybe JA.
Journal of Molecular Biology 2007 Nov; 375(5):1197.
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Crystal structure at 2.8 A of Huntingtin-interacting protein 1 (HIP1) coiled-coil domain reveals a charged surface suitable for HIP1 protein interactor (HIPPI).
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