Prkcb polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Prkcb.
Immunogen
A synthetic peptide corresponding to amino acids 660-673 of rat Prkcb.
Sequence
SFVNSEFLKPEVKS
Host
Rabbit
Reactivity
Rat
Specificity
These rabbit polyclonal antibodies were made against specific peptide regions of the PKC beta protein including beta I and beta II. Based on known homologies is expected to react with most mammalian species. These antibodies are useful in the screening of PKC beta proteins in various biological tissues and media upon expression of phorbitol esters.
Form
Liquid
Recommend Usage
Western Blot (1:250)
Immunocytochemistry (1:250)
Immunohistochemistry (1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.05% sodium azide
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
Enzyme Immunoassay
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Gene Info — Prkcb
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Publication Reference
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Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta.
Mischak H, Pierce JH, Goodnight J, Kazanietz MG, Blumberg PM, Mushinski JF.
The Journal of Biological Chemistry 1993 Sep; 268(27):20110.
Application:WB-Ce, Mouse, Mouse myeloid progenitor 39D cells.
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Tissue and cellular distribution of the extended family of protein kinase C isoenzymes.
Wetsel WC, Khan WA, Merchenthaler I, Rivera H, Halpern AE, Phung HM, Negro-Vilar A, Hannun YA.
Journal of Cellular Biology 1992 Apr; 117(1):121.
Application:ELISA, IHC-P, WB-Ti, Rat, Rat brain, heart, kidney, liver, lung, ovary, spleen, testes.
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Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta.
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