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Last updated: 2021/4/11
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RFA1/RFA2/RFA3 polyclonal antibody 

  • Catalog # : PAB13584
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant RFA1, RFA2, RFA3.
  • Immunogen:
  • Recombinant protein corresponding to Saccharomyces cerevisiae RFA1/RFA2/RFA3.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 70+30+14
  • Reactivity:
  • Yeast
  • Form:
  • Lyophilized
  • Recommend Usage:
  • ChIP
    Immunoprecipitation
    Western Blot (1:20000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from serum.
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with 50 uL sterile water, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Publication Reference
  • Applications
  • ChIP
  • Western Blot
  • Western Blot
  • TCA precipitated protein extracts from a wild type yeast strain (S. cerevisiae) were separated on 10% gel and transferred to a PVDF membrane. Antibody was used in different dilutions: 1: 5000 (1); 1: 10 000 (2); 1: 20 000 (3). Besides the bands for RFA1 and RFA2 an unspecific band was detected at ~150 kDa.
  • Immunoprecipitation
  • Application Image
  • ChIP
  • Immunoprecipitation
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