VTG polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Using Vitellogenin polyclonal antibody (Cat # PAB13582) at a 1 : 4,000 dilution.
(1) Plasma from male sole (load 0.11 uL of plasma).
(2) Plasma from vitellogenic female sole (load 0.11 uL of plasma).
(3) Plasma from estradiol-treated male sole (load 0.08 uL of plasma).
(4) Vitellogenin precipitate (load 0.83 uL of precipitate).
(5) Purified Vitellogenin (load 2 uL of purified preparation).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length native Vitellogenin (VTG).
Immunogen
Native purified Senegalese sole Vitellogenin.
Host
Rabbit
Reactivity
European seabass, Salmonids, Senegalese sole
Form
Lyophilized
Recommend Usage
Western Blot (1:5000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from serum
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Using Vitellogenin polyclonal antibody (Cat # PAB13582) at a 1 : 4,000 dilution.
(1) Plasma from male sole (load 0.11 uL of plasma).
(2) Plasma from vitellogenic female sole (load 0.11 uL of plasma).
(3) Plasma from estradiol-treated male sole (load 0.08 uL of plasma).
(4) Vitellogenin precipitate (load 0.83 uL of precipitate).
(5) Purified Vitellogenin (load 2 uL of purified preparation).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).
Guzman JM, Norberg B, Ramos J, Mylonas CC, Mananos EL.
General and Comparative Endocrinology 2008 Apr; 156(2):285.
Application：ELISA, WB, Fish, Fish plasma.
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Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis).
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