Using Vitellogenin polyclonal antibody (Cat # PAB13582) at a 1 : 4,000 dilution. (1) Plasma from male sole (load 0.11 uL of plasma). (2) Plasma from vitellogenic female sole (load 0.11 uL of plasma). (3) Plasma from estradiol-treated male sole (load 0.08 uL of plasma). (4) Vitellogenin precipitate (load 0.83 uL of precipitate). (5) Purified Vitellogenin (load 2 uL of purified preparation).