HSP90 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Lane 1 : HSP90 protein standard stressgen.
Lane 2 : Control (13°C) rainbow trout nuclear fraction.
Lane 3 : Heat shocked (25 °C) rainbow trout nuclear fraction.
Lane 4 : 24 hours post heat shock (13°C) rainbow trout nuclear fraction.
There will be some background bands in LMW range.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HSP90.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to Hsp90.
Host
Rabbit
Reactivity
Fish, Human, Rainbow trout
Form
Lyophilized
Recommend Usage
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from serum
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 100 uL deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Lane 1 : HSP90 protein standard stressgen.
Lane 2 : Control (13°C) rainbow trout nuclear fraction.
Lane 3 : Heat shocked (25 °C) rainbow trout nuclear fraction.
Lane 4 : 24 hours post heat shock (13°C) rainbow trout nuclear fraction.
There will be some background bands in LMW range. -
Publication Reference
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The role of hsp90 in 17alpha-ethynylestradiol-induced endocrine disruption in rainbow trout hepatocytes.
Osborne N, Sherry J, Rendell JL, Currie S.
Ecotoxicology and Environmental Safety 2007 Sep; 68(1):13.
Application：IF, WB, Fish, Rainbow trout hepatocytes.
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Development-dependent differences in intracellular localization of stress proteins (hsps) in rainbow trout, Oncorhynchus mykiss, following heat shock.
Jillian L. Rendell, Stephanie Fowler, Amanda Cockshutt and Suzanne Currie
Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics 2006 Jun; 1(2):238.
Application：IF, WB, FISH, FISH cardiomyocytes, hepatocytes, liver.
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The role of hsp90 in 17alpha-ethynylestradiol-induced endocrine disruption in rainbow trout hepatocytes.
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