Neisseria meningitidis Orf1/FrpD polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
Western Blotting analysis (reducing conditions) of recombinant protein Orf1/FrpD using polyclonal Orf1/FrpD polyclonal antibody (Cat # PAB13542).
Lane 1, 3, 5 : Cell lysate of Orf1/FrpD-transfected E. coli ; dilution (1) 0.5 ug/mL , (3) 0.2 ug/mL , (5) 0.1 ug/mL ; detection with Donkey anti-rabbit / HRP secondary antibody.
Lane 2, 4, 6 : Cell lysates of non-transfected E. coli (negative controls).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant Neisseria meningitidis Orf1/FrpD.
Immunogen
Recombinant His6 fusion protein corresponding to Neisseria meningitidis Orf1/ FrpD.
Host
Rabbit
Theoretical MW (kDa)
28-32
Specificity
This antibody reacts with Orf1 (FrpD), a 28-32 KDa Neisseria meningitidis outer membrane lipoprotein.
Form
Liquid
Concentration
1 mg/mL
Recommend Usage
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western Blotting analysis (reducing conditions) of recombinant protein Orf1/FrpD using polyclonal Orf1/FrpD polyclonal antibody (Cat # PAB13542).
Lane 1, 3, 5 : Cell lysate of Orf1/FrpD-transfected E. coli ; dilution (1) 0.5 ug/mL , (3) 0.2 ug/mL , (5) 0.1 ug/mL ; detection with Donkey anti-rabbit / HRP secondary antibody.
Lane 2, 4, 6 : Cell lysates of non-transfected E. coli (negative controls). -
Publication Reference
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Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance.
Oh-Hora M, Yamashita M, Hogan PG, Sharma S, Lamperti E, Chung W, Prakriya M, Feske S, Rao A.
Nature Immunology 2008 Apr; 9(4):432.
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STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER.
Grigoriev I, Gouveia SM, van der Vaart B, Demmers J, Smyth JT, Honnappa S, Splinter D, Steinmetz MO, Putney JW Jr, Hoogenraad CC, Akhmanova A.
Current Biology 2008 Feb; 18(3):177.
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Role of STIM and Orai proteins in the store-operated calcium signaling pathway.
Hewavitharana T, Deng X, Soboloff J, Gill DL.
Cell Calcium 2007 Aug; 42(2):173.
Application：IP, WB, Human, HEK 293 cells.
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Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance.
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