Procollagen Type III polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial Procollagen Type III.
Immunogen
Procollagen type III deprived of the C-terminal PIIICP peptide.
Host
Rabbit
Reactivity
Bovine, Human, Pig
Specificity
This antibody reacts with human and bovine procollagen type III (PIIIP) 100%; human and bovine collagen type III very low at RIA dilution <1:100. human and bovine procollagen type I <0.1% in RIA at 1:200 dilution.
Form
Lyophilized
Purification
Affinity purification
Recommend Usage
ELISA (1:100-1:200)
Immunohistochemistry (1:500)
Immunofluorescence (1:40)
Radioimmunoassay (>1:200)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS buffer, no BSA and preservative.
Storage Instruction
Store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Radioimmunoassay
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Publication Reference
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Four markers of collagen metabolism as possible indicators of disease in the adult respiratory distress syndrome.
Farjanel J, Hartmann DJ, Guidet B, Luquel L, Offenstadt G.
The American Review of Respiratory Disease 1993 May; 147(5):1091.
Application：ELISA, Human, Human bronchoalveolar lavage fluids.
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Serum type I collagen and N-terminal peptide of type III procollagen in patients with alcoholic liver disease: relationship to liver histology.
Trinchet JC, Hartmann DJ, Pateron D, Munz-Gotheil C, Callard P, Ville G, Beaugrand M.
Alcoholism, Clinical and Experimental Research 1992 Apr; 16(2):342.
Application：RIA, Human, Human serum.
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Four markers of collagen metabolism as possible indicators of disease in the adult respiratory distress syndrome.
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