Collagen I polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against native COL1A1/COL1A2.
Immunogen
Native purified COL1A1/COL1A2 from bovine skin.
Host
Rabbit
Reactivity
Bovine, Sheep
Specificity
This antibody reacts with bovine collagen type I 100%, bovine collagen II, IX, XI <0.1%; sheep collagen type I: 50 % (IHC estimated), bovine fibronectin <0.1%; human collagen type I <2%; chicken collagen type I <0.1% (estimates except sheep collagen I from solid phase RIA at dilution 1:500).
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:100-1:500)
Immunofluorescence (1:40)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from phosphate buffer
Storage Instruction
Store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Radioimmunoassay
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Gene Info — COL1A1
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Gene Info — COL1A2
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