SATB2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of SATB2 in mouse brain tissue lysate with SATB2 polyclonal antibody (Cat # PAB13377) at (A) 2 and (B) 4 ug/mL .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of SATB2 in human brain with SATB2 antibody at 5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SATB2.
Immunogen
A synthetic peptide corresponding to 16 amino acids near N-terminus of human SATB2. The immunogen is located within the first 50 amino acids of human SATB2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
At least two isoforms of SATB2 are known to exist. This antibody will not cross-react with SATB1.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
immunofluorescence (start at 20 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (starting at 5 ug/mL)
Western Blot (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide).
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of SATB2 in mouse brain tissue lysate with SATB2 polyclonal antibody (Cat # PAB13377) at (A) 2 and (B) 4 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of SATB2 in human brain with SATB2 antibody at 5 ug/mL .Immunofluorescence
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Gene Info — SATB2
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Interactome
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Disease
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Publication Reference
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SATB2 interacts with chromatin-remodeling molecules in differentiating cortical neurons.
Gyorgy AB, Szemes M, de Juan Romero C, Tarabykin V, Agoston DV.
The European Journal of Neuroscience 2008 Feb; 27(4):865.
Application：ChIP, IHC-Fr, WB-Ti, Rat, E18 rat cortex.
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SATB2 is a multifunctional determinant of craniofacial patterning and osteoblast differentiation.
Dobreva G, Chahrour M, Dautzenberg M, Chirivella L, Kanzler B, Fariñas I, Karsenty G, Grosschedl R.
Cell 2006 Jun; 125(5):971.
Application：IF, IHC-P, WB-Ti, Mouse, Mouse brain, embryo.
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Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential.
Dickinson LA, Kohwi-Shigematsu T.
Molecular and Cellular Biology 1995 Jan; 15(1):456.
Application：Func, WB, Human, Mouse, K-562 cells, Mouse thymus.
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SATB2 interacts with chromatin-remodeling molecules in differentiating cortical neurons.
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