VPS53 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of VPS53.
Immunogen
A synthetic peptide corresponding to internal region 18 amino acids of human VPS53.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of VPS53 in 293 cell lysate with VPS53 polyclonal antibody (Cat # PAB13370) at (A) 0.5 and (B) 1 ug/mL . -
Gene Info — VPS53
Entrez GeneID
55275Protein Accession#
EAW90658Gene Name
VPS53
Gene Alias
FLJ10979, HCCS1, MGC39512, hVps53L, pp13624
Gene Description
vacuolar protein sorting 53 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq
Other Designations
vacuolar protein sorting 53
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Interactome
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Disease
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Publication Reference
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Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes.
Perez-Victoria FJ, Mardones GA, Bonifacino JS.
Molecular Biology of the Cell 2008 Jun; 19(6):2350.
Application:IP, Human, HeLa cells.
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Identification of host proteins required for HIV infection through a functional genomic screen.
Brass AL, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ.
Science 2008 Jan; 319(5865):921.
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Characterization of the human GARP (Golgi associated retrograde protein) complex.
Liewen H, Meinhold-Heerlein I, Oliveira V, Schwarzenbacher R, Luo G, Wadle A, Jung M, Pfreundschuh M, Stenner-Liewen F.
Experimental Cell Research 2005 May; 306(1):24.
Application:IF, WB-Tr, Human, Monkey, COS-7, HEK 293 cells.
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Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes.
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