AMBRA1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AMBRA1.
Immunogen
A synthetic peptide corresponding to C-terminus 15 amino acids of human AMBRA1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of AMBRA1 in human heart tissue lysate with AMBRA1 polyclonal antibody (Cat # PAB13361) at (A) 0.5 and (B) 1 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of AMBRA1 in human brain with AMBRA1 polyclonal antibody (Cat # PAB13361) at 5 ug/mL . -
Gene Info — AMBRA1
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Interactome
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Publication Reference
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Ambra1 regulates autophagy and development of the nervous system.
Fimia GM, Stoykova A, Romagnoli A, Giunta L, Di Bartolomeo S, Nardacci R, Corazzari M, Fuoco C, Ucar A, Schwartz P, Gruss P, Piacentini M, Chowdhury K, Cecconi F.
Nature 2007 Jun; 447(7148):1121.
Application:IF, IP, WB-Tr, Human, 2FTGH cells.
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Autophagy as a cell death and tumor suppressor mechanism.
Gozuacik D, Kimchi A.
Oncogene 2004 Apr; 23(16):2891.
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Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells.
Kisen GO, Tessitore L, Costelli P, Gordon PB, Schwarze PE, Baccino FM, Seglen PO.
Carcinogenesis 1993 Dec; 14(12):2501.
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Ambra1 regulates autophagy and development of the nervous system.
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