DDIT4 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DDIT4.
Immunogen
A synthetic peptide corresponding to internal region 12 amino acids of human DDIT4.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of DDIT4 in human kidney tissue lysate with DDIT4 polyclonal antibody (Cat # PAB13343) at (A) 0.5 and (B) 1 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of DDIT4 in mouse kidney tissue with DDIT4 polyclonal antibody (Cat # PAB13343) at 5 ug/mL . -
Gene Info — DDIT4
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Interactome
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Pathway
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Publication Reference
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Epidermal growth factor-induced vacuolar (H+)-atpase assembly: a role in signaling via mTORC1 activation.
Xu Y, Parmar A, Roux E, Balbis A, Dumas V, Chevalier S, Posner BI.
The Journal of Biological Chemistry 2012 Jul; 287(31):26409.
Application:WB, Rat, Primary rat hepatocytes.
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Regulation of mTOR and cell growth in response to energy stress by REDD1.
Sofer A, Lei K, Johannessen CM, Ellisen LW.
Molecular and Cellular Biology 2005 Jul; 25(14):5834.
Application:WB, Mouse, MEFs.
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REDD1, a developmentally regulated transcriptional target of p63 and p53, links p63 to regulation of reactive oxygen species.
Ellisen LW, Ramsayer KD, Johannessen CM, Yang A, Beppu H, Minda K, Oliner JD, McKeon F, Haber DA.
Molecular Cell 2002 Nov; 10(5):995.
Application:IF, WB-Ce, WB-Tr, Human, Human keratinocytes, U-2 OS cells.
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Identification of a novel hypoxia-inducible factor 1-responsive gene, RTP801, involved in apoptosis.
Shoshani T, Faerman A, Mett I, Zelin E, Tenne T, Gorodin S, Moshel Y, Elbaz S, Budanov A, Chajut A, Kalinski H, Kamer I, Rozen A, Mor O, Keshet E, Leshkowitz D, Einat P, Skaliter R, Feinstein E.
Molecular and Cellular Biology 2002 Apr; 22(7):2283.
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Epidermal growth factor-induced vacuolar (H+)-atpase assembly: a role in signaling via mTORC1 activation.
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