TRIM25 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of TRIM25 in human thymus tissue lysate in (A) the absence and (B) absence of blocking peptide with TRIM25 polyclonal antibody (Cat # PAB13294) at 1 ug/mL .
Immunocytochemistry
Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 polyclonal antibody (Cat # PAB13294) at 5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TRIM25.
Immunogen
A synthetic peptide corresponding to N-terminus 15 amino acids of human TRIM25.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of TRIM25 in human thymus tissue lysate in (A) the absence and (B) absence of blocking peptide with TRIM25 polyclonal antibody (Cat # PAB13294) at 1 ug/mL .Immunocytochemistry
Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 polyclonal antibody (Cat # PAB13294) at 5 ug/mL . -
Gene Info — TRIM25
Entrez GeneID
7706Protein Accession#
NP_005073Gene Name
TRIM25
Gene Alias
EFP, RNF147, Z147, ZNF147
Gene Description
tripartite motif-containing 25
Omim ID
600453Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq
Other Designations
estrogen-responsive finger protein|tripartite motif protein TRIM25|zinc finger protein 147 (estrogen-responsive finger protein)|zinc finger protein-147
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Interactome
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Disease
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Publication Reference
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Analysis of estrogen-responsive finger protein expression in benign and malignant human breast.
Thomson SD, Ali S, Pickles L, Taylor J, Pace PE, Lymboura M, Shousha S, Coombes RC.
International Journal of Cancer 2001 Jan; 91(2):152.
Application：ICC, IF, IHC-P, WB-Tr, Human, Monkey, COS-1, MCF-7 cells, Human breast cancer.
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Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor alpha.
Orimo A, Inoue S, Minowa O, Tominaga N, Tomioka Y, Sato M, Kuno J, Hiroi H, Shimizu Y, Suzuki M, Noda T, Muramatsu M.
PNAS 1999 Oct; 96(21):12027.
Application：WB-Ti, Mouse, Uterus.
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Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
Inoue S, Orimo A, Hosoi T, Kondo S, Toyoshima H, Kondo T, Ikegami A, Ouchi Y, Orimo H, Muramatsu M.
PNAS 1993 Dec; 90(23):11117.
Application：WB-Ce, WB-Tr, Human, Monkey, COS-7, HBL-100 cells.
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Analysis of estrogen-responsive finger protein expression in benign and malignant human breast.
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