KIF5A polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of KIF5A in K-562 cell lysate with KIF5A polyclonal antibody (Cat # PAB13286) at (A) 0.5 and (B) 1 ug/mL .
Immunocytochemistry
Immunocytochemistry of KIF5A in K-562 cells with KIF5A polyclonal antibody (Cat # PAB13286) at 5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KIF5A.
Immunogen
A synthetic peptide corresponding to amino acids 330- 380 of human KIF5A.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Immunocytochemistry (start at 5 ug/mL)
Immunofluorescence (start at 20 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of KIF5A in K-562 cell lysate with KIF5A polyclonal antibody (Cat # PAB13286) at (A) 0.5 and (B) 1 ug/mL .Immunocytochemistry
Immunocytochemistry of KIF5A in K-562 cells with KIF5A polyclonal antibody (Cat # PAB13286) at 5 ug/mL .Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — KIF5A
Entrez GeneID
3798Protein Accession#
Q12840Gene Name
KIF5A
Gene Alias
D12S1889, MY050, NKHC, SPG10
Gene Description
kinesin family member 5A
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq
Other Designations
KIF5A variant protein|kinesin, heavy chain, neuron-specific
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Interactome
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Disease
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Publication Reference
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mRNA transport in dendrites: RNA granules, motors, and tracks.
Hirokawa N.
Journal of Neuroscience 2006 Jul; 26(27):7139.
Application：WB, Human, Human mammalian cells.
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Evidence of kinesin heavy chain (KIF5A) involvement in pure hereditary spastic paraplegia.
Fichera M, Lo Giudice M, Falco M, Sturnio M, Amata S, Calabrese O, Bigoni S, Calzolari E, Neri M.
Neurology 2004 Sep; 63(6):1108.
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Cloning and localization of a conventional kinesin motor expressed exclusively in neurons.
Niclas J, Navone F, Hom-Booher N, Vale RD.
Neuron 1994 May; 12(5):1059.
Application：IF, IHC-Fr, IP, WB-Ti, Rat, Rat brains, Rat tissues.
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mRNA transport in dendrites: RNA granules, motors, and tracks.
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