IL32 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IL32.
Immunogen
A synthetic peptide corresponding to C-terminus 15 amino acids of human IL32.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects all isoforms of IL-32.
Form
Liquid
Recommend Usage
Western Blot (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of IL32 in human spleen tissue lysate with IL32 polyclonal antibody (Cat # PAB13185) at (A) 5 and (B) 10 ug/mL shows two isoforms of IL-32.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of IL32 in human spleen tissue with IL32 polyclonal antibody (Cat # PAB13185) at 10 ug/mL . -
Gene Info — IL32
Entrez GeneID
9235Protein Accession#
AAH09401Gene Name
IL32
Gene Alias
IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd
Gene Description
interleukin 32
Omim ID
606001Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
natural killer cell transcript 4|natural killer cells protein 4|tumor necrosis factor alpha-inducing factor
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Interactome
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Publication Reference
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Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by in vitro cultured rheumatoid and osteoarthritis fibroblast-like synoviocytes.
Cagnard N, Letourneur F, Essabbani A, Devauchelle V, Mistou S, Rapinat A, Decraene C, Fournier C, Chiocchia G.
Eur Cytokine Netw 2005 Dec; 16(4):289.
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Interleukin-32: a cytokine and inducer of TNFalpha.
Kim SH, Han SY, Azam T, Yoon DY, Dinarello CA.
Immunity 2005 Jan; 22(1):131.
Application:Neutralization, WB, Human, Human A-549, COS-7, Wish cells, Recombinant protein.
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Identification of a novel gene expressed in activated natural killer cells and T cells.
Dahl CA, Schall RP, He HL, Cairns JS.
The Journal of Immunology 1992 Jan; 148(2):597.
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Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by in vitro cultured rheumatoid and osteoarthritis fibroblast-like synoviocytes.
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