Il31 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Il31.
Immunogen
A synthetic peptide corresponding to N-terminus 16 amino acids of mouse Il31.
Host
Rabbit
Reactivity
Mouse
Form
Liquid
Recommend Usage
Western Blot (2.5-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Il31 in Raw 264.7 cell lysate with Il31 polyclonal antibody (Cat # PAB13183) at (A) 2.5, (B) 5 and (C) 10 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Il31 in rat skeletal muscle tissue with Il31 polyclonal antibody (Cat # PAB13183) at 10 ug/mL . -
Gene Info — Il31
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Publication Reference
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IL-31 is associated with cutaneous lymphocyte antigen-positive skin homing T cells in patients with atopic dermatitis.
Bilsborough J, Leung DY, Maurer M, Howell M, Boguniewicz M, Yao L, Storey H, LeCiel C, Harder B, Gross JA.
The Journal of Allergy and Clinical Immunology 2006 Feb; 117(2):418.
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Predominant expression of the long isoform of GP130-like (GPL) receptor is required for interleukin-31 signaling.
Diveu C, Lak-Hal AH, Froger J, Ravon E, Grimaud L, Barbier F, Hermann J, Gascan H, Chevalier S.
Eur Cytokine Netw 2004 Oct; 15(4):291.
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Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice.
Dillon SR, Sprecher C, Hammond A, Bilsborough J, Rosenfeld-Franklin M, Presnell SR, Haugen HS, Maurer M, Harder B, Johnston J, Bort S, Mudri S, Kuijper JL, Bukowski T, Shea P, Dong DL, Dasovich M, Grant FJ, Lockwood L, Levin SD, LeCiel C, Waggie K, Day H, Topouzis S, Kramer J, Kuestner R, Chen Z, Foster D, Parrish-Novak J, Gross JA.
Nature Immunology 2004 Jul; 5(7):752.
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IL-31 is associated with cutaneous lymphocyte antigen-positive skin homing T cells in patients with atopic dermatitis.
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